The Ultimate Guide To high performance liquid chromatography
The Ultimate Guide To high performance liquid chromatography
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. Block diagram of the HPLC–MS. A 3 element mixture enters the HPLC. When element A elutes from the column, it enters the MS ion source and ionizes to form the parent ion and several other fragment ions.
각각 다른 산업 분야에 대한 자세한 정보 및 다양한 카테고리는 다음 써모 피셔 사이언티픽 학습 센터에서 산업 및 응용 과학 페이지를 확인하세요.
The realm of the height is mechanically detected by the computer. The computer also detect the retention time of that distinct element.
. Whenever we examine the chromatograms from these seven mobile phases we may possibly see that one or more presents an adequate separation, or we may possibly recognize a area in the solvent triangle where a separation is possible.
Next, a number of the compounds from the serum could take in too strongly for the stationary period, degrading the column’s performance. Ultimately, Though an HPLC can independent and examine complicated mixtures, an analysis is hard if the amount of constituents exceeds the column’s peak ability.
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규제 약물(마약, 합성 마약, 대마, 각성제, 향정신성 의약품, 아편양제제 등), 반도핑 관련(금지 물질, 금지 약물, 스테로이드 등), 약물 대사물
-hydroxybenzoic acid elutes much more gradually. While we could take care of entirely these two solutes working with cellular section that may be 16% v/v acetonitrile, we are unable to take care of them In the event the mobile period is 10% tetrahydrofuran.
The buy of elution of compounds with the column is ruled through the intensity of contact with the stationary period. The eluent with the separated chemical compounds flows previous the detector.
원하는 분석 결과를 얻기 위해서는 컬럼도 충분히 고려하고 선택하는 것이 좋습니다.
Conversely, a move amount that is too minimal could cause excessive band broadening. Check your circulation rate options and change them according to the recognized system.
Compounds from the check here sample partition among the stationary section plus the cell phase in partition chromatography. Compounds that has a more robust affinity for that stationary stage devote far more time interacting with it, resulting in slower elution in the column.
Right after loading the sample, the injector is turned check here into the inject posture, which redirects the cell section from the sample loop and onto the column.
. Example of a standard high-performance liquid chromatograph with insets showing the pumps that transfer the mobile stage from the system along with the plumbing accustomed to inject the sample in the cellular stage.